Phage-mediated bioluminescent detection ofBacillus anthracis
نویسندگان
چکیده
منابع مشابه
Diagnostic bioluminescent phage for the detection of Yersinia pestis
3 David A. Schofield*, Ian J. Molineux, and Caroline Westwater 4 5 6 Guild Associates, Inc., Charleston, South Carolina, 29407; Molecular Genetics and 7 Microbiology, Institute for Cellular and Molecular Biology, University of Texas at Austin, 8 Texas, 78712; 9 Department of Craniofacial Biology, Medical University of South Carolina, South Carolina, 1
متن کاملDiagnostic bioluminescent phage for detection of Yersinia pestis.
Yersinia pestis is the etiological agent of the plague. Because of the disease's inherent communicability, rapid clinical course, and high mortality, it is critical that an outbreak, whether it is natural or deliberate, be detected and diagnosed quickly. The objective of this research was to generate a recombinant luxAB ("light")-tagged reporter phage that can detect Y. pestis by rapidly and sp...
متن کاملLandscape phage, phage display, stripped phage, biosensors, detection, affinity reagent, nanotechnology, Salmonella typhimurium, Bacillus anthracis
Filamentous phage, such as fd used in this study, are thread-shaped bacterial viruses. Their outer coat is a tube formed by thousands equal copies of the major coat protein pVIII. We constructed libraries of random peptides fused to all pVIII domains and selected phages that act as probes specific for a panel of test antigens and biological threat agents. Because the viral carrier is infective,...
متن کامل'Bioluminescent' reporter phage for the detection of Category A bacterial pathogens.
Yersinia pestis and Bacillus anthracis are Category A bacterial pathogens that are the causative agents of the plague and anthrax, respectively. Although the natural occurrence of both diseases' is now relatively rare, the possibility of terrorist groups using these pathogens as a bioweapon is real. Because of the disease's inherent communicability, rapid clinical course, and high mortality rat...
متن کاملPhage display mediated immuno-PCR
Immuno-PCR (IPCR) is a powerful detection technology in immunological study and clinical diagnosis due to its ultrasensitivity. Here we introduce a new strategy termed phage display mediated immuno-PCR (PD-IPCR). Instead of utilization of monoclonal antibody (mAb) and chemically bond DNA that required in the conventional IPCR, a recombinant phage particle is applied as a ready reagent for IPCR ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2009
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2009.04332.x